SMAD2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB025507

 

Antibody CAB073546

 

ANTIBODY INFORMATION

Provider

R&D Systems
 Monash University MATF
 

Product name

MAB4037 HUPO 23 (anti-SMAD2) 

Host species

Mouse Mouse 

Clonality

mAb mAb 

Purity

Protein A/G Not known 

Released in version

5 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein Not known 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human testis shows nuclear positivity in cells of seminiferus ducts.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:150  

Literature conformity

Partly consistent with extensive gene/protein characterization data  

RNA consistency

Not done  

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line MCF7 shows positivity in nucleus, nucleoli & cytoplasm.
More information
 

Antibody dilution

 1:200 

Validation IF

 Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

52.3, 49.0, 48.7, 24.5, 15.2, 11.9, 11.7 52.3, 49.0, 48.7, 24.5, 15.2, 11.9, 11.7 

Antibody dilution

1:500 1:1000 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 
 

ANTIGEN VIEW

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 Not available