NDUFB4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA051739

 

Antibody HPA064441

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA051739 HPA064441 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

43 31 

Antigen sequence

MSFPKYKPSSLRTLPETLDPAEYNISPETRRAQAERLAIRAQL
 
LKREYLLQYNDPNRRGLIENPALLRWAYART
 

Matching transcripts

NDUFB4-001 - ENSP00000184266 [100%]
NDUFB4-002 - ENSP00000419578 [100%]
NDUFB4-006 - ENSP00000419128 [100%]
 NDUFB4-001 - ENSP00000184266 [100%]
NDUFB4-002 - ENSP00000419578 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:150  

Literature conformity

No avaliable gene/protein characterization data  

RNA consistency

Consistent with RNA expression data  

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in nuclear membrane, mitochondria & nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:62 1:96 

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

15.2, 14, 9.5 15.2, 14 

Antibody dilution

1:310 1:320 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2500 1:3850 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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NDUFB4-001
 
NDUFB4-002
 
NDUFB4-006