RPL18A

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA055259

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA055259 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

57 

Antigen sequence

RAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTT
KRPNTFF
 

Matching transcripts

RPL18A-001 - ENSP00000222247 [100%]
RPL18A-005 - ENSP00000471748 [100%]
RPL18A-008 - ENSP00000471360 [100%]
RPL18A-007 - ENSP00000473155 [89%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells,islets of Langerhans were negative.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:2500 

Literature conformity

No avaliable gene/protein characterization data 

RNA consistency

Not done 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A549 shows positivity in nucleus & nucleoli.
More information
 

Antibody dilution

1:56 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

20.8, 17.5, 16.7, 16.2 

Antibody dilution

1:280 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2250 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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RPL18A-001
 
RPL18A-005
 
RPL18A-007
 
RPL18A-008