TTR

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA002550

 

Antibody CAB002517

 

Antibody CAB062567

 

Antibody CAB073406

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Novocastra
 Atlas Antibodies
Sigma-Aldrich
 NCI-CPTC
 

Product name

HPA002550 NCL-PREp AMAb90649 CPTC-TTR-1 

Host species

Rabbit Rabbit Mouse Mouse 

Clonality

pAb pAb mAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Not known Protein A/G Protein A/G 

Released in version

3 1 12 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known Recombinant protein Recombinant protein 

Length (aa)

124    

Antigen sequence

GTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESG
ELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPR
RYTIAALLSPYSYSTTAVVTNPKE
 
 
 
 

Matching transcripts

TTR-001 - ENSP00000237014 [100%]
TTR-202 - ENSP00000479174 [94%]
    

Other gene match

    

ANTIBODY VALIDATION

         Immunohistochemistry

Image

    

Description

Immunohistochemical staining of human pancreas shows selective positivity in islets of Langerhans.
More information
 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
More information
 Immunohistochemical staining of human pancreas shows selective positivity in islets of Langerhans.
More information
 Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
More information
 

Retrieval method

HIER pH6 None HIER pH6 HIER pH6 

Antibody dilution

1:1000 1:600 1:8000 1:8000 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data Mainly consistent with RNA expression data Mainly consistent with RNA expression data Mainly consistent with RNA expression data 

         Immunofluorescence

Image

    

Description

Immunofluorescent staining of human cell line CACO-2 shows positivity in the Golgi apparatus.
More information
 Application not done for this antibody. Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:57    

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.    

         Western Blot

Image

    

Description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

15.9, 15.1 20.1, 15.9, 15.1 20.1, 15.9, 15.1 20.1, 15.9, 15.1 

Antibody dilution

1:250 1:500 1:500 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

    

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:3000    

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).    
 

ANTIGEN VIEW

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TTR-001
 
TTR-202