IL13RA1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA000293

 

Antibody HPA001008

 

Antibody HPA001587

 

Antibody HPA001795

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA000293 HPA001008 HPA001587 HPA001795 

Host species

Rabbit Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

14 5 2 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

123 123 122 122 

Antigen sequence

ETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIA
PETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAV
TELQCIWHNLSYMKCSWLPGRNT
 
ETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIA
PETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAV
TELQCIWHNLSYMKCSWLPGRNT
 
LTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSF
HNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYVQEAKCENPEFE
RNVENTSCFMVPGVLPDTLNTV
 
LTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSF
HNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYVQEAKCENPEFE
RNVENTSCFMVPGVLPDTLNTV
 

Matching transcripts

IL13RA1-001 - ENSP00000360730 [100%]
IL13RA1-002 - ENSP00000360705 [100%]
 IL13RA1-001 - ENSP00000360730 [100%]
IL13RA1-002 - ENSP00000360705 [100%]
 IL13RA1-001 - ENSP00000360730 [100%]
 IL13RA1-001 - ENSP00000360730 [100%]
 

Other gene match

    

ANTIBODY VALIDATION

         Immunohistochemistry

Image

    

Description

Application not done for this antibody. Immunohistochemical staining of human small intestine shows distinct nuclear positivity in glandular cells.
More information
 Immunohistochemical staining of human testis shows distinct nuclear positivity in basal cells of seminiferus ducts.
More information
 Application not done for this antibody. 

Retrieval method

 HIER pH6 HIER pH6  

Antibody dilution

 1:150 1:150  

Literature conformity

 Partly consistent with extensive gene/protein characterization data Not consistent with gene/protein characterization data  

RNA consistency

 Not done Not done  

         Immunofluorescence

Image

    

Description

Immunofluorescent staining of human cell line A-431 shows positivity in the Golgi apparatus & nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. Application not done for this antibody. Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:50   1:20 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.   Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

    

Description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

48.8, 31.7 48.8, 31.7 48.8 48.8 

Antibody dilution

1:500 1:500 1:500 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: No bands detected 

         Protein array

Image

    

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1000 1:2000 1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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IL13RA1-001
 
IL13RA1-002