MACROD1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA041031

 

Antibody HPA071075

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA041031 HPA071075 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

8 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

67 46 

Antigen sequence

SFLKGLSDKQREEHYFCKDFVRLKKIPTWKEMAKGVAVKVEEPRYKKDKQ
LNEKISLLRSDITKLEV
 
AEIVLATLREWLEQHKDKVDRLIICVFLEKDEDIYRSRLPHYFPVA
 

Matching transcripts

MACROD1-001 - ENSP00000255681 [100%]
 MACROD1-001 - ENSP00000255681 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human colon shows strong cytoplasmic positivity with granular pattern in glandular cells.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:325  

Literature conformity

Not consistent with gene/protein characterization data  

RNA consistency

Not done  

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line HeLa shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

 1:54 

Validation IF

 Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

  

Description

 Application not done for this antibody. 

Target mass (kDa)

35.5  

Antibody dilution

1:250  

Validation WB

Non-supportive: Only bands not corresponding to the predicted size  

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:2000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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MACROD1-001