ZNF300

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA051777

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA051777 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

60 

Antigen sequence

EEPWIIKGDISNWIYPDEYQADGRQDRKSNLHNSQSCILGTVSFHHKILK
GVTRDGSLCS
 

Matching transcripts

ZNF300-201 - ENSP00000274599 [100%]
ZNF300-202 - ENSP00000392593 [100%]
ZNF300-203 - ENSP00000397178 [100%]
ZNF300-001 - ENSP00000377773 [97%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:100 

Literature conformity

No avaliable gene/protein characterization data 

RNA consistency

Not done 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

70.4, 68.7, 64.5 

Antibody dilution

1:140 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:15000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

? »
 
 
 
ZNF300-001
 
ZNF300-201
 
ZNF300-202
 
ZNF300-203