HIST1H2BA

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042205

 

Antibody HPA043013

 

Antibody CAB011603

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Abcam plc
 

Product name

HPA042205 HPA043013 ab23913 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

12 12 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Synthetic peptide 

Length (aa)

27 26  

Antigen sequence

MGIMNSFVNDIFERIAGEASRLAHYNK
 
KESYSIYVYKVLKQVHPDTGISSKAM
 
 

Matching transcripts

HIST1H2BA-001 - ENSP00000274764 [85%]
 HIST1H2BA-001 - ENSP00000274764 [96%]
  

Other gene match

HIST1H2BJ - ENSG00000124635 [100%]
HIST1H2BD - ENSG00000158373 [100%]
HIST1H2BC - ENSG00000180596 [100%]
HIST2H2BE - ENSG00000184678 [100%]
HIST1H2BL - ENSG00000185130 [96%]
HIST3H2BB - ENSG00000196890 [96%]
HIST1H2BK - ENSG00000197903 [100%]
HIST2H2BF - ENSG00000203814 [100%]
HIST1H2BN - ENSG00000233822 [100%]
H2BFS - ENSG00000234289 [96%]
HIST1H2BM - ENSG00000273703 [100%]
HIST1H2BG - ENSG00000273802 [100%]
HIST1H2BE - ENSG00000274290 [100%]
HIST1H2BO - ENSG00000274641 [100%]
HIST1H2BH - ENSG00000275713 [100%]
HIST1H2BB - ENSG00000276410 [100%]
HIST1H2BF - ENSG00000277224 [100%]
HIST1H2BI - ENSG00000278588 [100%]
 HIST1H2BJ - ENSG00000124635 [100%]
HIST1H2BD - ENSG00000158373 [96%]
HIST1H2BC - ENSG00000180596 [96%]
HIST2H2BE - ENSG00000184678 [100%]
HIST1H2BL - ENSG00000185130 [96%]
HIST3H2BB - ENSG00000196890 [100%]
HIST1H2BK - ENSG00000197903 [96%]
HIST2H2BF - ENSG00000203814 [96%]
HIST1H2BN - ENSG00000233822 [96%]
H2BFS - ENSG00000234289 [96%]
HIST1H2BM - ENSG00000273703 [96%]
HIST1H2BG - ENSG00000273802 [96%]
HIST1H2BE - ENSG00000274290 [96%]
HIST1H2BO - ENSG00000274641 [100%]
HIST1H2BH - ENSG00000275713 [96%]
HIST1H2BB - ENSG00000276410 [100%]
HIST1H2BF - ENSG00000277224 [96%]
HIST1H2BI - ENSG00000278588 [96%]
  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.
More information
 Immunohistochemical staining of human stomach, upper shows strong nuclear positivity in glandular cells.
More information
 Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:200 1:800 1:3000 

Literature conformity

Partly consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Not consistent with RNA expression data Not consistent with RNA expression data Mainly consistent with RNA expression data 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

18.8, 14.8, 14.2, 14, 13.9 18.8, 14.8, 14.2, 14, 13.9 14.2 

Antibody dilution

1:250 1:250 1:500 

Validation WB

Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Current setup is not applicable due to low RNA count 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000 1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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HIST1H2BA-001