MUM1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA048063

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA048063 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

108 

Antigen sequence

APLEELAYRRSLRVALDVLSEGSIWSQESSAGTGRADRSLRGKPMEHVSS
PCDSNSSSLPRGDVLGSSRPHRRRPCVQQSLSSSFTCEKDPECKVDHKKG
LRKSENPR
 

Matching transcripts

MUM1-001 - ENSP00000467083 [100%]
MUM1-009 - ENSP00000467287 [100%]
MUM1-014 - ENSP00000394925 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human heart muscle shows distinct staining of intercalated disc in myocytes.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:75 

Literature conformity

Not consistent with gene/protein characterization data 

RNA consistency

Not done 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line RT4 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:84 

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

80.8, 78.8, 28.8 

Antibody dilution

1:200 

Validation WB

Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3350 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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MUM1-001
 
MUM1-009
 
MUM1-014