AMDHD2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA041184

 

Antibody HPA041321

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA041184 HPA041321 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

8 8 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

91 119 

Antigen sequence

FITHLFNAMLPFHHRDPGIVGLLTSDRLPAGRCIFYGMIADGTHTNPAAL
RIAHRAHPQGLVLVTDAIPALGLGNGRHTLGQQEVEVDGLT
 
PTLVTSPPEVYHKVVPQIPVKSGGPHGAGVLGLHLEGPFISREKRGAHPE
AHLRSFEADAFQDLLATYGPLDNVRIVTLAPELGRSHEVIRALTARGICV
SLGHSVADLRAAEDAVWSG
 

Matching transcripts

AMDHD2-001 - ENSP00000307481 [100%]
AMDHD2-003 - ENSP00000391596 [100%]
AMDHD2-004 - ENSP00000293971 [100%]
AMDHD2-011 - ENSP00000456898 [100%]
AMDHD2-012 - ENSP00000457398 [100%]
AMDHD2-002 - ENSP00000457136 [91%]
 AMDHD2-001 - ENSP00000307481 [100%]
AMDHD2-003 - ENSP00000391596 [100%]
AMDHD2-004 - ENSP00000293971 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human adrenal gland shows strong cytoplasmic in glandular cells.
More information
 Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:450 1:100 

Literature conformity

No avaliable gene/protein characterization data No avaliable gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data Mainly consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleoli & cytoplasm.
More information
 Application not done for this antibody. 

Antibody dilution

1:189  

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

63.6, 46.9, 43.7, 31.9, 31.5, 28.5 63.6, 46.9, 43.7 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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AMDHD2-001
 
AMDHD2-002
 
AMDHD2-003
 
AMDHD2-004
 
AMDHD2-011
 
AMDHD2-012