SLMAP

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA002357

 

Antibody HPA002358

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA002357 HPA002358 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

2 2 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

76 150 

Antigen sequence

TTDAQMDEQDLNEPLAKVSLLKALLEEERKAYRNQVEESTKQIQVLQAQL
QRLHIDTENLREEKDSEITSTRDELL
 
SEYEKEITSLQNSFQLRCQQCEDQQREEATRLQGELEKLRKEWNALETEC
HSLKRENVLLSSELQRQEKELHNSQKQSLELTSDLSILQMSRKELENQVG
SLKEQHLRDSADLKTLLSKAENQAKDVQKEYEKTQTVLSELKLKFEMTEQ
 

Matching transcripts

SLMAP-004 - ENSP00000391886 [100%]
SLMAP-016 - ENSP00000418218 [93%]
SLMAP-018 - ENSP00000420697 [93%]
 SLMAP-001 - ENSP00000295951 [100%]
SLMAP-005 - ENSP00000412829 [100%]
SLMAP-006 - ENSP00000389978 [100%]
SLMAP-008 - ENSP00000419543 [100%]
SLMAP-013 - ENSP00000398661 [100%]
SLMAP-014 - ENSP00000412945 [100%]
SLMAP-016 - ENSP00000418218 [100%]
SLMAP-201 - ENSP00000295952 [100%]
SLMAP-004 - ENSP00000391886 [87%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human smooth muscle shows strong nuclear and cytoplasmic positivity in smooth muscle cells.
More information
 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:250 1:250 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in endoplasmic reticulum.
More information
 

Antibody dilution

1:51 1:49 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

37.1, 36.3, 12.6 95.2, 93.2, 90.8, 50.3, 47.3, 42, 37.1, 36.3 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). 
 

ANTIGEN VIEW

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SLMAP-001
 
SLMAP-004
 
SLMAP-005
 
SLMAP-006
 
SLMAP-008
 
SLMAP-013
 
SLMAP-014
 
SLMAP-016
 
SLMAP-018
 
SLMAP-201