PHAX

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA059057

 

Antibody HPA059630

 

Antibody HPA070326

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA059057 HPA059630 HPA070326 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

14 14 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

84 72 84 

Antigen sequence

ESQEHTKDLDKELDEYMHGGKKMGSKEEENGQGHLKRKRPVKDRLGNRPE
MNYKGRYEITAEDSQEKVADEISFRLQEPKKDLI
 
SDSDMTVAPSDRPLQLPKVLGGDSAMRAFQNTATACAPVSHYRAVESVDS
SEESFSDSDDDSCLWKRKRQKC
 
ESQEHTKDLDKELDEYMHGGKKMGSKEEENGQGHLKRKRPVKDRLGNRPE
MNYKGRYEITAEDSQEKVADEISFRLQEPKKDLI
 

Matching transcripts

PHAX-001 - ENSP00000297540 [100%]
 PHAX-001 - ENSP00000297540 [100%]
 PHAX-001 - ENSP00000297540 [100%]
 

Other gene match

   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Application not done for this antibody. Application not done for this antibody. Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells and glial cells.
More information
 

Retrieval method

  HIER pH6 

Antibody dilution

  1:1000 

Literature conformity

  Consistent with extensive gene/protein characterization data 

RNA consistency

  Mainly consistent with RNA expression data 

         Immunofluorescence

Image

   

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles & nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus.
More information
 Application not done for this antibody. 

Antibody dilution

1:200 1:27  

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody.  

         Western Blot

Image

   

Description

   

Target mass (kDa)

44.4 44.4 44.4 

Antibody dilution

1:700 1:90 1:770 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Non-supportive: Only bands not corresponding to the predicted size Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:8400 1:1000 1:9250 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PHAX-001