PRAP1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035360

 

Antibody HPA038713

 

Antibody HPA052451

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035360 HPA038713 HPA052451 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 7 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Recombinant protein fragment 

Length (aa)

71 66 63 

Antigen sequence

VRMHLYPADFRFDKPKKSRSPASAEAPGDSGERSEGEAMEICSEPVAASP
APAGERRIYRHRSVSELFLKP
 
PILPGTKAWMETEDTLGRVLSPEPDHDSLYHPPPEEDQGEERPRLWVMPN
HQVLLGPEEDQDHIYH
 
AVPAPKVPIKMQVKHWPSEQDPEKAWGARVVEPPEKDDQLVVLFPVQKPK
LLTTEEKPRGQGR
 

Matching transcripts

PRAP1-201 - ENSP00000357542 [87%]
 PRAP1-001 - ENSP00000416126 [98%]
PRAP1-201 - ENSP00000357542 [98%]
 PRAP1-001 - ENSP00000416126 [100%]
PRAP1-201 - ENSP00000357542 [100%]
 

Other gene match

ZNF511 - ENSG00000198546 [100%]
   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human duodenum shows strong positivity in a subset of cells.
More information
 Immunohistochemical staining of human duodenum shows strong positivity in a subset of glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:50 1:1500 1:350 

Literature conformity

Partly consistent with gene/protein characterization data Consistent with gene/protein characterization data Partly consistent with gene/protein characterization data 

RNA consistency

Not consistent with RNA expression data Consistent with RNA expression data Mainly consistent with RNA expression data 

         Western Blot

Image

   

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407941)
More information
 

Target mass (kDa)

35.2, 29.4, 28.3 35.2, 17.2 35.2, 17.2 

Antibody dilution

1:250 1:250 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

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Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:30000 1:5150 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PRAP1-001
 
PRAP1-201