EVPL

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA053969

 

Antibody CAB002220

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Novocastra
 

Product name

HPA053969 NCL-ENVO 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Not known 

Released in version

13 1 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Not known 

Length (aa)

89  

Antigen sequence

DVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIAEHNILQKEIDAYGQ
QLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQ
 
 

Matching transcripts

EVPL-001 - ENSP00000301607 [100%]
EVPL-002 - ENSP00000465630 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human breast shows moderate membranous and cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human esophagus shows strong positivity in squamous epithelial cells.
More information
 

Retrieval method

HIER pH6 HIER pH9 

Antibody dilution

1:700 1:20 

Literature conformity

Consistent with extensive gene/protein characterization data Partly consistent with extensive gene/protein characterization data 

RNA consistency

Consistent with RNA expression data Mainly consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line MCF7 shows positivity in intermediate filaments.
More information
 Application not done for this antibody. 

Antibody dilution

1:71  

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody.  

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

233.8, 231.6 233.8, 231.6, 12.6 

Antibody dilution

1:360 1:500 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:2850  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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EVPL-001
 
EVPL-002