ZNF540

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA003444

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA003444 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

3 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

135 

Antigen sequence

LSSEKDIHEISLSKESIIEKSKTLRLKGSIFRNEWQNKSEFEGQQGLKER
SISQKKIVSKKMSTDRKRPSFTLNQRIHNSEKSCDSHLVQHGKIDSDVKH
DCKECGSTFNNVYQLTLHQKIHTGEKSCKCEKCGK
 

Matching transcripts

ZNF540-001 - ENSP00000467118 [100%]
ZNF540-002 - ENSP00000324598 [100%]
ZNF540-003 - ENSP00000465503 [100%]
ZNF540-004 - ENSP00000343768 [100%]
ZNF540-009 - ENSP00000466274 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:250 

Literature conformity

Consistent with gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in vesicles & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:40 

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

 

Description

 

Target mass (kDa)

77.1, 73.7, 64.3 

Antibody dilution

1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ZNF540-001
 
ZNF540-002
 
ZNF540-003
 
ZNF540-004
 
ZNF540-009