RAB43

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA046840

 

Antibody HPA060085

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA046840 HPA060085 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

39 38 

Antigen sequence

SDLSELREVSLAEAQSLAEHYDILCAIETSAKDSSNVEE
 
EAFLRVATELIMRHGGPLFSEKSPDHIQLNSKDIGEGW
 

Matching transcripts

RAB43-001 - ENSP00000319781 [100%]
RAB43-002 - ENSP00000376981 [100%]
RAB43-003 - ENSP00000376985 [100%]
RAB43-004 - ENSP00000376982 [100%]
RAB43-005 - ENSP00000376984 [100%]
 RAB43-001 - ENSP00000319781 [100%]
RAB43-002 - ENSP00000376981 [100%]
RAB43-003 - ENSP00000376985 [100%]
RAB43-004 - ENSP00000376982 [100%]
RAB43-005 - ENSP00000376984 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Application not done for this antibody. Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:1300 

Literature conformity

 Partly consistent with extensive gene/protein characterization data 

RNA consistency

 Mainly consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line PC-3 shows positivity in plasma membrane.
More information
 Application not done for this antibody. 

Antibody dilution

1:200  

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

23.3 23.3 

Antibody dilution

1:1440 1:690 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:11500 1:8250 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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RAB43-001
 
RAB43-002
 
RAB43-003
 
RAB43-004
 
RAB43-005