PEAK1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA026313

 

Antibody HPA026517

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA026313 HPA026517 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

86 90 

Antigen sequence

DGQSICGELSIQEHCENKPVIIGWNRNRAALSQKPLNNNNEDDEGISHVP
KPYGNNDSAKKMSDNNNGLTEVLKEIAGLDTAPQIR
 
EPNYESPSSNNQDKDSSQASKSSIKVPETHKAVLALRLEEKDGKIAVQTE
KEESKASTDVAGQAVTINLVPTEEQAKPYRVVNLEQPLCK
 

Matching transcripts

PEAK1-001 - ENSP00000452796 [100%]
PEAK1-005 - ENSP00000453924 [100%]
PEAK1-006 - ENSP00000457880 [100%]
PEAK1-201 - ENSP00000309230 [100%]
 PEAK1-001 - ENSP00000452796 [100%]
PEAK1-005 - ENSP00000453924 [100%]
PEAK1-006 - ENSP00000457880 [100%]
PEAK1-201 - ENSP00000309230 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human stomach shows distinct cytoplasmic and membrane positivity in glandular cells.
More information
 Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:10 1:1500 

Literature conformity

No avaliable gene/protein characterization data No avaliable gene/protein characterization data 

RNA consistency

Not done Not done 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-251 MG shows positivity in plasma membrane, cytoplasm, focal adhesion sites & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

 1:200 

Validation IF

 Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

193.1, 115.2, 72 193.1, 115.2, 72 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PEAK1-001
 
PEAK1-005
 
PEAK1-006
 
PEAK1-201