GCSAM

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA002473

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA002473 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

2 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

124 

Antigen sequence

HIAEGCFCLPWKKILIFEKRQDSQNENERMSSTPIQDNVDQTYSEELCYT
LINHRVLCTRPSGNSAEEYYENVPCKAERPRESLGGTETEYSLLHMPSTD
PRHARSPEDEYELLMPHRISSHFL
 

Matching transcripts

GCSAM-001 - ENSP00000309487 [100%]
GCSAM-007 - ENSP00000419485 [100%]
GCSAM-005 - ENSP00000420603 [91%]
GCSAM-008 - ENSP00000419913 [86%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in reaction center cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:500 

Literature conformity

Partly consistent with extensive gene/protein characterization data 

RNA consistency

Mainly not consistent with RNA expression data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

21.2, 21, 19.2, 14.9 

Antibody dilution

1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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GCSAM-001
 
GCSAM-005
 
GCSAM-007
 
GCSAM-008