NDUFA4L2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA055323

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA055323 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

25 

Antigen sequence

VCWDRNNPEPWNKLGPNDQYKFYSV
 

Matching transcripts

NDUFA4L2-001 - ENSP00000450664 [81%]
NDUFA4L2-201 - ENSP00000377411 [81%]
 

Other gene match

NDUFA4 - ENSG00000189043 [100%]
 

ANTIBODY VALIDATION

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line MCF7 shows positivity in mitochondria.
More information
 

Antibody dilution

1:6 

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

10, 9.4 

Antibody dilution

1:80 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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NDUFA4L2-001
 
NDUFA4L2-201