MIB2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA027871

 

Antibody CAB037055

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 SIGMA
 

Product name

HPA027871 M5823 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

7 14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide 

Length (aa)

89  

Antigen sequence

VGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENK
SSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLL
 
 

Matching transcripts

MIB2-001 - ENSP00000348081 [100%]
MIB2-003 - ENSP00000428264 [100%]
MIB2-006 - ENSP00000367984 [100%]
MIB2-008 - ENSP00000367980 [100%]
MIB2-016 - ENSP00000426103 [100%]
MIB2-019 - ENSP00000426128 [100%]
MIB2-021 - ENSP00000427680 [100%]
MIB2-022 - ENSP00000428660 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:200 1:400 

Literature conformity

Partly consistent with gene/protein characterization data Partly consistent with gene/protein characterization data 

RNA consistency

Mainly not consistent with RNA expression data Mainly not consistent with RNA expression data 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

115.9, 115.4, 114.3, 108.9, 105.1, 99.5, 89.7, 82.1 115.9, 115.4, 114.3, 108.9, 105.1, 99.5, 89.7, 82.1, 34.8, 19.7, 16.8 

Antibody dilution

1:250 1:500 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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MIB2-001
 
MIB2-003
 
MIB2-006
 
MIB2-008
 
MIB2-016
 
MIB2-019
 
MIB2-021
 
MIB2-022