NDUFA1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA029768

 

Antibody HPA054359

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA029768 HPA054359 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

14 11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

39 39 

Antigen sequence

HRFTNGGKEKRVAHFGYHWSLMERDRRISGVDRYYVSKG
 
HRFTNGGKEKRVAHFGYHWSLMERDRRISGVDRYYVSKG
 

Matching transcripts

NDUFA1-001 - ENSP00000360492 [100%]
 NDUFA1-001 - ENSP00000360492 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Application not done for this antibody. Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:400 

Literature conformity

 Partly consistent with extensive gene/protein characterization data 

RNA consistency

 Mainly consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line HEK 293 shows positivity in cytoplasm & mitochondria.
More information
 Application not done for this antibody. 

Antibody dilution

1:24  

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.  

         Western Blot

Image

  

Description

 Target too small/large to be analyzed with the present setup 

Target mass (kDa)

8.1 8.1 

Antibody dilution

1:250  

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Target too small/large to be analyzed with the present setup 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:6250 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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NDUFA1-001