CDK5R1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA000252

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA000252 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

14 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

104 

Antigen sequence

FITPANVVFLYMLCRDVISSEVGSDHELQAVLLTCLYLSYSYMGNEISYP
LKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPHYFTQVFSDLKNESG
QEDK
 

Matching transcripts

CDK5R1-001 - ENSP00000318486 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line SH-SY5Y shows positivity in vesicles & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:50 

Validation IF

Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

34.1 

Antibody dilution

1:250 

Validation WB

Uncertain: No bands detected 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CDK5R1-001